Re: Tux X Tux = NORMAL?

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: In just about every tuxedo file off the CD I have found one female that will throw non-tux fry.  Once you have identified her you can name her "non-tux" and use her to produce normal bodied yellows, blues, silvers and greys (clear and grass).  The female I have on file at this time is F03 (although I don't remember if she is always this number with every reload).  I have used her to produce non-tux strains of all the colors (clear and grass) represented in the tuxedo phenotype. I have proven to myself that she is also sky blue (which I find recessive to all other colors) which make her very versitile as a "brood mother".  She saved me a whole bunch of out-crosses. Thank you AZ. 

: If you use a "proven" tuxedo female in an out-cross with a non-tux male all the F1 males will be tuxedo.  The F2 will segregate out into tux and non-tux males.  The ratio will depend on the number of males AZ gives you in the brood (probably 50/50). This means some (probably half) of the females will also be non-tux.  Mating a tux male with his sisters will show you which of his sisters are tux. 

: If you mate a tux male with a "known" non-tux female, all of the F1 males will be non-tux.  The F2 generation of a randomly picked pair from the F1 will yield 2 or 3 tux males (if AZ gives you enough males in the brood). I have found that if I use the same F2 tux male to father the F3-F5 generations (back-crossing) I can easily fix the tux trait into the strain without doing the "tux-sister search" routine.  

: This strongly suggests that the tux trait is sex-linked via the female and is recessive via the male. Others out there can supply the scientific jargon, I just know what happens when I mate this with that, that with this, ad infinitum......  

: Don


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